Ceramic materials lead to underestimated DNA quantifications : a method for reliable measurements

In the context of investigating cell-material interactions or of material-guided generation of tissues, DNA quantification represents an elective method to precisely assess the number of cells attached or embedded within different substrates. Nonetheless, nucleic acids are known to electrostatically bind to ceramics, a class of materials commonly employed in orthopaedic implants and bone tissue engineering scaffolds. This phenomenon is expected to lead to a relevant underestimation of the DNA amount, resulting in erroneous experimental readouts. The present work aims at *lpar;i) investigating the effects of DNA-ceramic bond occurrence on DNA quantification, and (ii) developing a method to reliably extract and accurately quantify DNA in ceramic-containing specimens. A cell-free model was adopted to study DNA-ceramic binding, highlighting an evident DNA loss (up to 90%) over a wide range of DNA/ceramic ratios (w/w). A phosphate buffer-based (800 mM) enzymatic extraction protocol was developed and its efficacy in terms of reliable DNA extraction and measurement was confirmed with commonly used fluorometric assays, for various ceramic substrates. The proposed buffered DNA extraction technique was validated in a cell-based experiment showing 95% DNA retrieval in a cell seeding experiment, demonstrating a 3.5-fold increase in measured DNA amount as compared to a conventional enzymatic extraction protocol. In conclusion, the proposed phosphate buffer method consistently improves the DNA extraction process assuring unbiased analysis of samples and allowing accurate and sensitive cell number quantification on ceramic containing substrates

A Tentative Description of Z_c,b States in Terms of Metastable Feshbach Resonances

We attempt a description of the recently discovered Z_{c,b} states in terms of Feshbach resonances arising from the interaction between the `closed' subspace of hadrocharmonium levels and the `open' one of open-charm/beauty thresholds. We show how the neutrality of the X(3872) might be understood in this scheme and provide a preliminary explanation of the pattern of the measured total widths of X,Z_{c,b}.Comment: To appear in the proceedings of The 6th International Workshop on Charm Physics (CHARM 2013

Large N.G. explosion and fire involving several buried utility networks

This paper describes an accident (explosion and natural gas fire) that occurred in Turin (Italy), in which power distribution cables, tramway network feeding cables and a gas pipe were involved. The described accident is particularly interesting because it occurred in the town centre and lasted several hours, producing a very high risk for the population. Fortunately, nobody was injured, but 120 people were evacuated for 24 h. The sequence of events is described, the involved facilities are examined and the physical processes which led to the different top events are discussed. Actually, starting from a modest event (600 V electric cable loss of insulation), which most likely lasted for months, the aforementioned accident was reached in a crescendo of domino effects. This sequence has been represented by an ISD in which the failure of the different protection systems is highlighted. These protection systems were mostly based upon the strict respect of procedures both in the installation and in the following maintenance of the different utilities. These aspects have been also briefly devised in the light of Italian and foreign regulations concerning the problem of the coexistence of buried utilities

A new look at scalar mesons

Light scalar mesons are found to fit rather well a diquark-antidiquark description. The resulting nonet obeys mass formulae which respect, to a good extent, the OZI rule. OZI allowed strong decays are reasonably reproduced by a single amplitude describing the switch of a qbar-q pair, which transforms the state into two colourless pseudoscalar mesons. Predicted heavy states with one or more quarks replaced by charm or beauty are briefly described; they should give rise to narrow states with exotic quantum numbers.Comment: 4 pages, 1 figure, misprints corrected, references added, accepted for publication in Phys. Rev. Let

Which affects affect the use of new technologies? Italian adaptation of the Internet Motive Questionnaire for Adolescents (IMQ-A) and criterion validity with problematic use and body dissatisfaction

Given the negative role of problematic use of new technological devices (NTD) in behavioral and psychological domains, the aim of the study is the Italian adaptation and validation of the Internet Motive Questionnaire for Adolescents (IMQ-A) in order to understand the motivation for the use of NTD. A total of 769 students 10-19 aged (M = 13.22, SD = 1.56) completed the IMQ-A, the Collins Figures Rating Scale, and two measures regarding the problematic NTD use, focused on overuse during the night and during meals. The IMQ-A showed adequate internal consistency with regard to its four subscales: Coping (α = .84), Social (α = .80), Enhancement (α = .80), and Conformity (α = .68) motives. However, with regard to factorial structure, a threefactor model (excluding Conformity subscale) showed slightly better fit indices than the original model. Coping motive was correlated with problematic NTD use and succeeded in predicting higher scores in body dissatisfaction as evidence of criterion-related and external validity. The Italian adaptation of the IMQ-A can be useful in both research and clinical fields, in order to propose alternative strategies for coping to users and to improve emotion regulation facets

Source complexity of the May 20, 2012, MW 5.9, Ferrara (Italy) event

A Mw 3.9 foreshock on May 19, 2012, at 23:13 UTC, was followed at 02:03 on May 20, 2012, by a Mw 5.9 earthquake that hit a densely populated area in the Po Plain, west of the city of Ferrara, Italy (Figure 1). Over the subsequent 13 days, six Mw >5 events occurred; of these, the most energetic was a Mw 5.8 earthquake on May 29, 2012, 12 km WSW of the main shock. The tragic balance of this sequence was 17 casualties, hundreds of injured, and severe damage to the historical and cultural heritage of the area. From a seismological point of view, the 2012 earthquake was not an outstanding event in its regional context. The same area was hit in 1996 by a Mw 5.4 earthquake [Selvaggi et al. 2001], and previously in 1986 and in 1967 (DBMI11) [Locati et al. 2011]. The most destructive historical event was the 1570, Imax 8 event, which struck the town of Ferrara [Guidoboni et al. 2007, Rovida et al. 2011]. The 2012 seismic sequence lasted for several weeks and probably developed on a well-known buried thrust fault [Basili et al. 2008, Toscani et al. 2009, DISS Working Group 2010], at depths between 2 km and 10-12 km

Electronic, optical and thermal properties of the hexagonal and fcc Ge2Sb2Te5 chalcogenide from first-principle calculations

We present a comprehensive computational study on the properties of face-centered cubic and hexagonal chalcogenide Ge2Sb2Te5. We calculate the electronic structure using density functional theory (DFT); the obtained density of states (DOS) compares favorably with experiments, also looking suitable for transport analysis. Optical constants including refraction index and absorption coefficient capture major experimental features, aside from an energy shift owed to an underestimate of the band gap that is typical of DFT calculations. We also compute the phonon DOS for the hexagonal phase, obtaining a speed of sound and thermal conductivity in good agreement with the experimental lattice contribution. The calculated heat capacity reaches ~ 1.4 x 106 J/(m3 K) at high temperature, in agreement with experimental data, and provides insight into the low-temperature range (< 150 K), where data are unavailable.Comment: 19 pages, 8 figure

Early brain damage affects body schema and person perception abilities in children and adolescents with spastic diplegia

Early brain damage leading to cerebral palsy is associated to core motor impairments and also affects cognitive and social abilities. In particular, previous studies have documented specific alterations of perceptual body processing and motor cognition that are associated to unilateral motor deficits in hemiplegic patients. However, little is known about spastic diplegia (SpD), which is characterized by motorial deficits involving both sides of the body and is often associated to visuospatial, attentional, and social perception impairments. Here, we compared the performance of a sample of 30 children and adolescents with SpD (aged 7-18 years) and of a group of age-matched controls with typical development (TD) at two different tasks tapping on body representations. In the first task, we tested visual and motor imagery abilities as assessed, respectively, by the object-based mental rotation of letters and by the first-person transformations for whole-body stimuli. In the second task, we administered an inversion effect/composite illusion task to evaluate the use of configural/holistic processing of others' body. Additionally, we assessed social perception abilities in the SpD sample using the NEPSY-II battery. In line with previously reported visuospatial deficits, a general mental imagery impairment was found in SpD patients when they were engaged in both object-centered and first-person mental transformations. Nevertheless, a specific deficit in operating an own-body transformation emerged. As concerns body perception, while more basic configural processing (i.e., inversion effect) was spared, no evidence for holistic (i.e., composite illusion) body processing was found in the SpD group. NEPSY-II assessment revealed that SpD children were impaired in both the theory of mind and affect recognition subtests. Overall, these findings suggested that early brain lesions and biased embodied experience could affect higher-level motor cognition and perceptual body processing, thus pointing to a strict link between motor deficits, body schema alterations, and person processing difficulties